Lentiviral Reporter Constructs for Fluorescence Tracking of the Temporospatial Pattern of Smad3 Signaling | Zendy

Christina H. Stuelten | Zendy; Anil K. Kamaraju | Zendy; Lalage M. Wakefield | Zendy; Anita B. Roberts | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Lentiviral Reporter Constructs for Fluorescence Tracking of the Temporospatial Pattern of Smad3 Signaling

Author(s) -

Christina H. Stuelten,

Anil K. Kamaraju,

Lalage M. Wakefield,

Anita B. Roberts

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112549

Subject(s) - green fluorescent protein , biology , endogeny , microbiology and biotechnology , signal transduction , in vivo , reporter gene , cell , metastasis , cell signaling , cancer research , cancer , gene , gene expression , genetics , biochemistry

Canonical TGF-beta is involved in cell differentiation, tissue maintenance, and wound healing, but also plays a central role in the pathogenesis of diseases such as cancer Here we describe a lentivirus-based reporter vector system expressing green fluorescent protein (GFP) or red fluorescent protein (RFP) under the control of a Smad3-responsive element (CAGA)12 that allows observation of the temporospatial pattern of endogeneous Smad3-mediated signaling on a cellular level. Use of this method will be valuable to identify cells with active Smad3 signaling and investigate the role of endogenous Smad3 signaling in complex systems such as co-cultures in vitro, or in tumors during tumor cell invasion and metastasis in vivo.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research