In-Fusion™ Assembly: Seamless Engineering of Multidomain Fusion Proteins, Modular Vectors, and Mutations | Zendy

Baogong Zhu | Zendy; Guifang Cai | Zendy; Emily O. Hall | Zendy; Gordon J. Freeman | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

In-Fusion™ Assembly: Seamless Engineering of Multidomain Fusion Proteins, Modular Vectors, and Mutations

Author(s) -

Baogong Zhu,

Guifang Cai,

Emily O. Hall,

Gordon J. Freeman

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112536

Subject(s) - restriction enzyme , dna , computational biology , fusion protein , plasmid , modular design , fusion , mutagenesis , protein engineering , in vitro recombination , biology , genetics , recombinant dna , computer science , mutation , molecular cloning , enzyme , gene , biochemistry , programming language , complementary dna , linguistics , philosophy

In-Fusion can join any two pieces of DNA that have a 15-bp overlap at their ends. The result is equivalent to a recombination event at the ends of the DNAs. The 15-bp overlap may be engineered by inclusion in primers used to PCR amplify a segment of DNA. Originally described for inserting one piece of DNA into a restriction enzyme-digested plasmid, we have found In-Fusion can join four or more pieces of DNA in a single reaction. We used this insight to construct seamless fusion proteins, modular vectors with readily interchangeable segments, and novel mutagenesis strategies. Replacement In-Fusion can be used to delete any desired DNA segment in a plasmid and replace it with any desired new DNA segment without limitations on position or size.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research