Protein III-Based Single-Chain Antibody Phage Display Using Bacterial Cells Bearing an Additional Genome of a Gene-III-Lacking Helper Phage | Zendy

Bizhi Shi | Zendy; Huamao Wang | Zendy; Guo Sheng-rong | Zendy; Yuhong Xu | Zendy; Zonghai Li | Zendy; Jianren Gu | Zendy

Open Access

Protein III-Based Single-Chain Antibody Phage Display Using Bacterial Cells Bearing an Additional Genome of a Gene-III-Lacking Helper Phage

Author(s) -

Bizhi Shi,

Huamao Wang,

Guo Sheng-rong,

Yuhong Xu,

Zonghai Li,

Jianren Gu

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112461

Subject(s) - phagemid , phage display , antibody , biology , genome , gene , microbiology and biotechnology , antigen , computational biology , bacteriophage , genetics , escherichia coli

We present herein a novel method of pIII-based antibody phage display using Hpd3cells--bacterial cells bearing the genome of a gene-III-lacking helper phage (VCSM13d3). A high level of single-chain variable fragments (scFvs) was displayed in the consequent phagemid particles using Hpd3cells to rescue the phagemid encoding scFv-pIII. Hpd3cells considerably improved the specific enrichment factor when used for constructing an immunized antibody library. In addition, using Hpd3cells could overcome pill resistance and can contribute to the efficient enrichment of specific binding antibodies from a phage display library, thereby increasing the chance of obtaining more diverse antibodies specific for target antigens.

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