Application of Protein-Fragment Complementation Assays in Cell Biology | Zendy

Ingrid Remy | Zendy; Stephen W. Michnick | Zendy

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Application of Protein-Fragment Complementation Assays in Cell Biology

Author(s) -

Ingrid Remy,

Stephen W. Michnick

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112396

Subject(s) - protein fragment complementation assay , complementation , fragment (logic) , protein–protein interaction , biology , enzyme , target protein , computational biology , microbiology and biotechnology , biochemistry , gene , computer science , programming language , phenotype

We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.

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