5′-Tailed Sequencing Primers Improve Sequencing Quality of PCR Products | Zendy

Jonas Binladen | Zendy; M. Thomas | Zendy; M. Thomas P. Gilbert | Zendy; Paula F. Campos | Zendy; Eske Willerslev | Zendy

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5′-Tailed Sequencing Primers Improve Sequencing Quality of PCR Products

Author(s) -

Jonas Binladen,

M. Thomas,

M. Thomas P. Gilbert,

Paula F. Campos,

Eske Willerslev

Publication year - 2007

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112316

Subject(s) - sanger sequencing , electropherogram , dna sequencing , biology , massive parallel sequencing , genetics , polymerase chain reaction , primer (cosmetics) , sequencing by ligation , ancient dna , computational biology , dna , genomic library , base sequence , gene , medicine , chemistry , organic chemistry , population , environmental health , electrophoresis

Primer DetailsThe primers were designed to the desired length by adding a by adding a neutral 40-bp sequence (5′-AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAA-3′) and a poly(C) tail to the 5′ end of the PCR primer (1,2). The neutral sequence is a randomly generated sequence not matching any sequence when a simple Basic Local Alignment Search Tool (BLAST) search is done in GenBank

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