Sensitive Cell Viability Assay for Use in Drug Screens and for Studying the Mechanism of Action of Drugs in Dictyostelium Discoideum | Zendy

Junxia Min | Zendy; Priya Sridevi | Zendy; Stephen Alexander | Zendy; Hannah Alexander | Zendy

Open Access

Sensitive Cell Viability Assay for Use in Drug Screens and for Studying the Mechanism of Action of Drugs in Dictyostelium Discoideum

Author(s) -

Junxia Min,

Priya Sridevi,

Stephen Alexander,

Hannah Alexander

Publication year - 2006

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112260

Subject(s) - dictyostelium discoideum , viability assay , mutant , dictyostelium , high throughput screening , cytotoxicity , drug discovery , biology , computational biology , phenotypic screening , mechanism of action , drug , genetic screen , cell , microbiology and biotechnology , biochemistry , in vitro , pharmacology , phenotype , gene

In order to increase the effectiveness of Dictyostelium discoideum as a lead genetic model for drug discovery, a luminescence-based assay has been adapted and standardized for sensitive and rapid cell viability measurements. The applicability of the assay was demonstrated by measuring the cytotoxicity of several drugs in wild-type and mutant cells. The robustness and ease of the assay demonstrate that it can be used in high-throughput applications such as drug or mutant screens. Conclusions from these studies are applicable to evaluating cell viability assays in other systems as well.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research