Improved DNA Methylation Analysis Via Enrichment of Demethylated Cells Expressing an X-Inactivated Transgene | Zendy

Tammy W. Vallender | Zendy; Bruce T. Lahn | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Improved DNA Methylation Analysis Via Enrichment of Demethylated Cells Expressing an X-Inactivated Transgene

Author(s) -

Tammy W. Vallender,

Bruce T. Lahn

Publication year - 2006

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112246

Subject(s) - demethylation , dna demethylation , demethylating agent , dna methylation , transgene , microbiology and biotechnology , biology , gene , dna , methylation , gene expression , gene expression profiling , genetics

The demethylating drug 5-aza-2'-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we describe a method that uses the reactivation of an X-inactivated green fluorescent protein (GFP) transgene as a marker to enrich for cells that have undergone drug-induced demethylation. By combining it with microarray gene expression profiling, we demonstrate the method's utility in identifying genes activated by global DNA demethylation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research