Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin | Zendy

Tushar Khare | Zendy; Carol S. Giometti | Zendy

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Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin

Author(s) -

Tushar Khare,

Carol S. Giometti

Publication year - 2006

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112171

Subject(s) - biotinylation , avidin , monomer , chemistry , biotin , biochemistry , chromatography , biology , microbiology and biotechnology , computational biology , polymer , organic chemistry

commercially available. This reagent permits recovery of biotinylated proteins and peptides using milder elution conditions, thus preserving native protein conformation and enzyme activity (5). In experiments to biotinylate proteins using biotin conjugates with different spacer arm lengths, we have observed differential protein recovery when immobilized polymeric or monomeric avidin is used. Our results illustrate the importance of selecting the optimal combination of biotinylation and avidin reagents for the recovery of the proteins of interest in proteomics studies.

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