One-Step High-Throughput Assay for Quantitative Detection of β-Galactosidase Activity in Intact Gram-Negative Bacteria, Yeast, and Mammalian Cells | Zendy

Faustino Vidal-Aroca | Zendy; Michele Giannattasio | Zendy; Elisa Brunelli | Zendy; Alessandro Vezzoli | Zendy; Paolo Plevani | Zendy; Marco Muzi-Falconi | Zendy; Giovanni Bertoni | Zendy

Open Access

One-Step High-Throughput Assay for Quantitative Detection of β-Galactosidase Activity in Intact Gram-Negative Bacteria, Yeast, and Mammalian Cells

Author(s) -

Faustino Vidal-Aroca,

Michele Giannattasio,

Elisa Brunelli,

Alessandro Vezzoli,

Paolo Plevani,

Marco Muzi-Falconi,

Giovanni Bertoni

Publication year - 2006

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112145

Subject(s) - bacteria , yeast , biology , gram , gram negative bacteria , high throughput screening , microbiology and biotechnology , saccharomyces cerevisiae , biochemistry , escherichia coli , genetics , gene

(shaded bars; left scale) are presented beside β-gal activity units determined on the indicated strains in the same conditions by ONPG-based assays (empty bars; right scale), performed using the traditional Miller method for bacteria (3) and crude protein extracts for yeast (1). MUG, 4-methylumbelliferyl β-d-galactopyranoside; ONPG, o-nitrophenyl-β-d-galactopyranoside; β-gal; β-galactosidase. BENCHMARKS

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