Whole-mount BrdU staining of proliferating cells by DNase treatment: application to postnatal mammalian retina
Author(s) -
Andrei V. Tkatchenko
Publication year - 2006
Publication title -
biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000112094
Subject(s) - bromodeoxyuridine , biology , cell cycle , thymidine , cell growth , proliferation marker , proliferating cell nuclear antigen , dna replication , microbiology and biotechnology , staining , deoxyuridine , dna , immunohistochemistry , cell , immunology , genetics
Whole Mount Brdu Staining Assessing cellular proliferation in whole-mount preparations is typically done using BrdU labeling. Unfortunately, traditional methods usually rely upon treatment with hyd...
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