Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis | Zendy

Lan-Szu Chou | Zendy; Cindy Meadows | Zendy; Carl T. Wittwer | Zendy; Elaine Lyon | Zendy

Open Access

Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis

Author(s) -

Lan-Szu Chou,

Cindy Meadows,

Carl T. Wittwer,

Elaine Lyon

Publication year - 2005

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112050

Subject(s) - genotyping , oligonucleotide , salt lake , nucleic acid , locked nucleic acid , melting temperature , melting curve analysis , taqman , microbiology and biotechnology , computational biology , genetics , dna , chemistry , biology , genotype , polymerase chain reaction , materials science , gene , composite material , paleontology , structural basin

With a frequency of 1 in 1000 bp, single nucleotide polymorphisms (SNPs) are used to study complex inherited diseases (1,2). A universal concern in genotyping techniques is that rare variants may interfere. Melting analysis addresses this concern and is a homogeneous and simple method for genotyping (3,4). For example, the 2-probe/2-fluorophore system HybProbe

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