Open AccessConcentration and isolation of DNA from biological fluids by agarose gel isotachophoresis
Author(s) -
В. Н. Кондратова,
Ol'ga I. Serd'uk,
В. П. Шелепов,
A. V. Lichtenstein
Publication year - 2005
Publication title -
biotechniques/biotechniques
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.617
H-Index - 131
eISSN - 1940-9818
pISSN - 0736-6205
DOI - 10.2144/000112020
Subject(s) - isotachophoresis , agarose , chromatography , nucleic acid , dna , electrophoresis , chemistry , isolation (microbiology) , agarose gel electrophoresis , biological fluids , polynucleotide , biology , biochemistry , electrolyte , electrode , microbiology and biotechnology
Isotachophoresis is an electrophoretic method of separation of charged substances. The method is characterized by a discontinuous buffer system, constant velocity of separated molecules, and the distribution of separated components in the form of narrow concentrated bands located one right after another. As a rule, isotachophoresis is not used for the separation of nucleic acids because the mobility of polynucleotides in this system does not depend on their size. However, this circumstance proved to be very useful for the quantitative isolation of heterogeneous DNA fragments from biological fluids, for gene diagnostics of cancer in particular. The proposed method of agarose gel isotachophoresis of DNA has been used for the isolation of blood DNA and its successful PCR analysis.