Minimally invasive method for murine brain fixation | Zendy

Kenneth D. Eichenbaum | Zendy; Joseph W. Eichenbaum | Zendy; Ahmed Fadiel | Zendy; Douglas C. Miller | Zendy; Necdet Demir | Zendy; Frederick Naftolin | Zendy; Arnold Stern | Zendy; Paul H. Pevsner | Zendy

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Minimally invasive method for murine brain fixation

Author(s) -

Kenneth D. Eichenbaum,

Joseph W. Eichenbaum,

Ahmed Fadiel,

Douglas C. Miller,

Necdet Demir,

Frederick Naftolin,

Arnold Stern,

Paul H. Pevsner

Publication year - 2005

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/000112003

Subject(s) - fixation (population genetics) , brain tissue , perfusion , electron microscope , biomedical engineering , dilation (metric space) , cytoplasm , magnification , perfusion scanning , microscopy , pathology , biology , medicine , microbiology and biotechnology , radiology , computer science , optics , biochemistry , physics , mathematics , combinatorics , gene , computer vision

Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas for study using electron microscopy. This perfusion fixation technique provides both faster tissue harvesting capability and higher quality tissue preservation, without the artifacts of brain swelling and ventricular dilation observed in direct cardiac perfusion. Acute, discrete change in brain tissue can be studied.

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