Effective Flow Cytometric Phenotyping of Cells Using Minimal Amounts of Antibody | Zendy

Deepak Sharma | Zendy; Mark R. Eichelberg | Zendy; Jill D. Haag | Zendy; Amanda L. Meilahn | Zendy; Matthew J. Muelbl | Zendy; Kathy Schell | Zendy; Bart M. G. Smits | Zendy; Michael N. Gould | Zendy

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Effective Flow Cytometric Phenotyping of Cells Using Minimal Amounts of Antibody

Author(s) -

Deepak Sharma,

Mark R. Eichelberg,

Jill D. Haag,

Amanda L. Meilahn,

Matthew J. Muelbl,

Kathy Schell,

Bart M. G. Smits,

Michael N. Gould

Publication year - 2012

Publication title -

biotechniques

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.617

H-Index - 131

eISSN - 1940-9818

pISSN - 0736-6205

DOI - 10.2144/0000113854

Subject(s) - flow cytometry , antibody , microbiology and biotechnology , antigen , biology , intracellular , cell , staining , immunology , biochemistry , genetics

Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate results for the detection of up to seven parameters for activation induced-proliferation, cell cycle analysis, and phenotyping of cell-surface and intracellular antigens.

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