Open AccessMembrane Polar Lipid Changes in Zoysiagrass Rhizomes and Their Potential Role in Freezing Tolerance
Author(s) -
Qi Zhang,
Jack D. Fry,
C. B. Rajashekar,
Dale J. Bremer,
M. C. Engelke
Publication year - 2009
Publication title -
journal of the american society for horticultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.408
H-Index - 80
eISSN - 2327-9788
pISSN - 0003-1062
DOI - 10.21273/jashs.134.3.322
Subject(s) - rhizome , phosphatidic acid , freezing tolerance , botany , diacylglycerol kinase , chemistry , cold tolerance , phosphatidylethanolamine , zoysia japonica , phosphatidylcholine , horticulture , biology , biochemistry , phospholipid , membrane , protein kinase c , gene , enzyme
Cell membranes play an integral role in freezing tolerance. The objectives of this study were to quantify polar lipids in cold-tolerant ‘Meyer’ zoysiagrass ( Zoysia japonica ) and cold-sensitive ‘Cavalier’ zoysiagrass ( Zoysia matrella ) and to evaluate their potential role in freezing tolerance. Grasses were acclimated outside and sampled once monthly between October and January to determine freezing tolerance and lipid composition in rhizomes. Lowest LT 50 s (temperature resulting in 50% survival) were observed in November for ‘Cavalier’ (−8.5 and −9.6 °C in 2005 and 2006, respectively) and December for ‘Meyer’ (−16.2 and −15.4 °C in 2005 and 2006, respectively). The most abundant lipids in zoysiagrass rhizomes were monogalactosyl diacylglycerol, digalactosyl diacylglycerol, phosphatidylcholine, phosphatidylethanolamine, and phosphatidic acid, which comprised 90% of the polar lipids. Differences in lipid contents and double bond indices (DBI) were detected between ‘Meyer’ and ‘Cavalier’ during cold acclimation, but there were no consistent relationships between lipid classes or DBI and freezing tolerance in zoysiagrass.