Open AccessMolecular Markers for the Scab Resistance (Vf) Region in Apple
Author(s) -
Minou Hemmat,
N. F. Weeden,
Herb S. Aldwinckle,
Susan K. Brown
Publication year - 1998
Publication title -
journal of the american society for horticultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.408
H-Index - 80
eISSN - 2327-9788
pISSN - 0003-1062
DOI - 10.21273/jashs.123.6.992
Subject(s) - bulked segregant analysis , rapd , biology , venturia inaequalis , locus (genetics) , genetics , genetic marker , population , malus , apple scab , gene , genetic linkage , gene mapping , marker assisted selection , botany , cultivar , chromosome , genetic diversity , fungicide , demography , sociology
Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the V f gene in apple ( Malus sp.) that confers resistance to five races of apple scab [ Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to V f . The most tightly linked marker in the test population, S5 2500 , was cloned and sequenced. A linkage map of the V f region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for V f by multiplexing the two markers closely flanking the V f locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.