DNA Fingerprint Analysis of Roses | Zendy

Alexander Vainstein | Zendy; Hagit BenMeir | Zendy

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DNA Fingerprint Analysis of Roses

Author(s) -

Alexander Vainstein,

Hagit BenMeir

Publication year - 1994

Publication title -

journal of the american society for horticultural science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.408

H-Index - 80

eISSN - 2327-9788

pISSN - 0003-1062

DOI - 10.21273/jashs.119.5.1099

Subject(s) - dna profiling , biology , microsatellite , genetics , dna , genotype , restriction enzyme , microbiology and biotechnology , allele , genetic marker , gene

Mini- and microsatellite probes were hybridized to DNA of 24 rose ( Rosa × hybrida ) genotypes. The resultant DNA fingerprints were shown to be genotype-specific, thereby enabling cultivar identification at the DNA level. Restriction enzyme Dra I yielded the most informative band patterns. Full-sib family analysis of DNA fingerprints revealed 32 parental-specific bands out of the 128 observed in the parents. These bands were revealed cumulatively by phage (M13), human (33.6), and oligonucleotide (GACA) 4 probes. Only one pair of these loci was found to be allelic, and no linked pairs were detected in the progeny analyzed. The probability of two offspring from this cross having identical DNA fingerprints was calculated to be 2 × 10 -8 .

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