Open AccessSomatic Embryogenesis in Habanero Pepper (C. chinense Jacq.) From Cell Suspensions
Author(s) -
Patricia Yolanda Zapata-Castillo,
Adriana-Canto Flick,
Guadalupe López-Puc,
Anabel Solís-Ruiz,
Felipe Barahona-Pérez,
Nancy SantanaBuzzy,
Lourdes G. IglesiasAndreu
Publication year - 2007
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.42.2.329
Subject(s) - somatic embryogenesis , pepper , capsicum chinense , hypocotyl , callus , explant culture , biology , botany , murashige and skoog medium , horticulture , in vitro , biochemistry
To induce the somatic embryogenesis of Habanero pepper, different culture media and different types of explants (node, internode, hypocotyl, half seeds, and fruit segments) were evaluated. For the induction of embryogenic callus, 9.05 μ m of 2,4-dichlorofenoxiacetic acid, 3% sucrose, and 0.8% gelrite were added to the basic MS medium over a period of 30 days at 25 ± 2 °C under continuous light (40–50 μmol·m 2 ·s −1 ). Once the callus formed, they were transferred to liquid medium using the same induction formulation. Somatic embryogenesis only occurred from explants of hypocotyl and in the presence of 3.4 μ m thidiazuron. This constitutes the first proposal of a protocol for the “induction of somatic embryogenesis in Habanero pepper ( Capsicum chinense Jacq.) from cell suspensions.”