Open Access(74) Effect of Temperature on Axillary Bud Formation and Polyamine Contents of Nonbranching Chrysanthemum
Author(s) -
Yeun Joo Huh,
Seoung Youl Choi,
Hak Ki Shin,
Chun Ho Pak
Publication year - 2005
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.40.4.1014c
Subject(s) - spermine , polyamine , axillary bud , spermidine , putrescine , horticulture , chemistry , shoot , botany , biology , biochemistry , in vitro , tissue culture , enzyme
Nonbranching chrysanthemums [ Dendranthema × grandiflorum (Ramat.) Kitamura] are preferred because they require less labor in disbudding. High temperature is responsible for this phenotype of not having axillary buds or poor lateral shoot development. This study attempted to find out the effect of temperature and identify the involvement of endogenous polyamine contents in axillary bud formation of nonbranching chrysanthemum cv. Iwanohakusen. Plants were treated at 22, 26, 30, 34, and 38 °C for 9 hours midday for 2 months. Polyamine content [putrescine (Put), spermidine (Spd), spermine (Spm)] was analyzed 1 month after treatment and axillary buds were counted when the flowers opened. Results revealed that viable axillary buds decreased remarkably at 30 and 34°C. It was also found out that not only low temperature, but also the excessively high temperature of 38 °C induced axillary bud formation. Exposure to 38 °C increased the Put contents and resulted in high Put/(Spd + Spm) ratio as 22 °C, 26 °C. Temperature of 30, 34 °C lowered Put/(Spd + Spm) ratio. Results further showed that not polyamine contents, but polyamine ratio (Put/Spd + Spm) or transformation of Put to Spd and Spm may be involved in the axillary bud formation in nonbranching chrysanthemum.