Open AccessTemporary Relationship Between C2 H4 Evolution and C2 H4 Recptor Genes
Author(s) -
Theophanes Solomos,
Prabodh Kumar Trivedi,
Mehar Hasan Asif,
Neelam Pathak,
Autar K. Mattoo
Publication year - 2004
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.39.4.846c
Subject(s) - climacteric , ripening , receptor , chemistry , gene expression , gene , messenger rna , endocrinology , medicine , biology , microbiology and biotechnology , botany , biochemistry , menopause
`Granny Smith' apples were stored at 1 °C, 5 °C and 20 °C, then treated at the preclimacteric stage with 2 ppm MCP, various O 2 concentrations, and MCP + low O 2 . All the treatments greatly retarded the onset of the C 2 H 4 climacteric, and hence ripening. MCP + low O 2 was much more effective than were the applications of MCP and low O 2 singly. Even at 20 °C, 4.04 kPa O 2 inhibited the rise in C 2 H 4 evolution for 145 d. Neither low O 2 nor MCP inhibited the System 1 C 2 H 4 evolution. The suppression of the climacteric rise in C 2 H 4 evolution was accompanied by a strong inhibition of the accumulation of ERS1 C 2 H 4 receptor and ACS transcripts. On the other hand, ETR1 receptor was constitutively expressed. When climacteric fruits were treated with MCP, and with low O 2 + MCP the rate of C 2 H 4 evolution decreased sharply. This occurred simultaneously with a decrease in ERS1 mRNA. Moreover, the decrease in ERS1 mRNA paralleled the decrease in C 2 H 4 evolution. The data thus indicate that the initiation and sustainment of the C 2 H 4 climacteric requires the presence of functional C 2 H 4 receptors. The expression of ETR2 and ERS2 is also under investigation.