Open AccessResponse of Sweetpotato Nodal Cultures to Low Temperature Stress
Author(s) -
Tianna W. Weathington,
DeviPrasad V. Potluri
Publication year - 2004
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.39.4.771e
Subject(s) - cultivar , proline , shoot , axillary bud , biology , ipomoea , horticulture , carbohydrate , botany , in vitro , tissue culture , amino acid , biochemistry
Axillary bud cultures of sweetpotato Ipomoea batatus L. [cultivars comensal and salyboro] were propagated in vitro. Nodal cultures of these were grown at different temperatures ranging from 10 °C to 30 °C at 5 °C intervals from the time of axillary bud transfer to 10 weeks of growth in a controlled growth chamber. After 10 weeks of growth, morphological and physiological parameters were measured including shoot height, number of nodes and branches, levels of proline, soluble carbohydrate and protein. There was not much difference in the cultures grown at 25 and 30 °C but temperatures lower than 25 °C were inhibitory to both cultivars, though the effects were more pronounced in salyboro than comensal. Salyboro grew slower and exhibited leaf discoloration, weak stems, and poor root growth. Proline levels increased in both cultivars and the increase was higher in shoot than root due to low temperature stress. Soluble carbohydrates and proteins increased in comensal, maintaining the carbohydrate protein ratio, but decreased in salyboro. The results suggest that the cultivar comensal may tolerate cooler temperatures and the cultivar salyboro is susceptible to them.