Open AccessRegeneration In Vitro From the Hypocotyl of Cucumis Species Produces Almost Exclusively Diploid Shoots, and Does Not Require Light
Author(s) -
Sebahattin Çürük,
G. Ananthakrishnan,
S.M. Singer,
Xiaodi Xia,
C. Elman,
David Nestel,
Selim Çetiner,
Victor Gaba
Publication year - 2003
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.38.1.105
Subject(s) - hypocotyl , cucumis , biology , cotyledon , melon , explant culture , shoot , botany , basal shoot , regeneration (biology) , cucurbitaceae , horticulture , in vitro , microbiology and biotechnology , biochemistry
Hypocotyl explants of three cultivars of melon ( Cucumis melo L.) (cvs. Revigal, Topmark and Kirkagac), and a cucumber ( C. sativus L. cv. Taoz) rapidly directly regenerated multiple shoots on Murashige and Skoog medium augmented with 4.4 μ m benzyladenine. Regeneration from the hypocotyl resulted in nearly 100% diploid shoots, whereas regeneration from the cotyledons resulted in 40% to 70% polyploid regenerants. Regeneration from cotyledon explants of melon cv. Revigal required light, whereas regeneration from hypocotyl explants of melon cv. Revigal occurred in both light and darkness. Direct regeneration also occurred from the hypocotyl of cucumber cv. Taoz in both light and darkness, even though cotyledonary explants did not regenerate buds or shoots under the same conditions. This is the first report of regeneration from the Cucumis genus producing a fully diploid plant population.