Open AccessDevelopment of the Press Extraction Method for Plug Substrate Analysis: Quantitative Relationships Between Solution Extraction Techniques
Author(s) -
Holly L. Scoggins,
Douglas A. Bailey,
Paul V. Nelson
Publication year - 2001
Publication title -
hortscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.518
H-Index - 90
eISSN - 2327-9834
pISSN - 0018-5345
DOI - 10.21273/hortsci.36.5.918
Subject(s) - extraction (chemistry) , peat , spark plug , coir , nutrient , substrate (aquarium) , chemistry , seedling , chromatography , materials science , horticulture , composite material , biology , ecology , organic chemistry , engineering , aerospace engineering
Substrate electrical conductivity (EC), pH, and nutrient content should be monitored frequently during seedling plug production. Current testing methods are either complicated, unsuited to plug production, or interpretation standards do not exist. This study compares the press extraction (PE) method developed at North Carolina State Univ. with the saturated media extract (SME) method and the 1 substrate: 2 water suspension method (1:2). These solution extraction methods were applied to plug trays containing peat-based germination mix treated with four levels of fertilizer. Two sample sizes of 20 or 60 plug cells were used to determine if the smaller, less destructive sample size would produce satisfactory results. Resulting pH values varied within 0.3 units among methods, but variability in EC and nutrient content was greater. The PE method resulted in the highest values for EC, NH 4 + -N, NO 3 - -N, K, Ca, and Mg while sample size had little effect on analyses. The three extraction methods were then compared on peat- and coir-based substrates. Within substrates, pH, EC, and nutrients tested were similar for the PE and the SME. The coir extract had a higher pH and much higher levels of K and Na than did the peat extract but was lower in N, P, Ca, and Mg. Overall, fairly strong correlations among testing methods were found, especially between the SME and PE.