Open AccessDetermination of the Molar Extinction Coefficients of the B800 and B850 Absorption Bands in Light-harvesting Complexes 2 Derived from Three Purple Photosynthetic Bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides, and Phaeospirillum molischianum by Extraction of Bacteriochlorophyll a
Author(s) -
Yoshitaka Saga,
Keiya Hirota
Publication year - 2016
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.32.801
Subject(s) - rhodobacter sphaeroides , chemistry , bacteriochlorophyll , molar absorptivity , rhodobacter , absorption (acoustics) , purple bacteria , rhodospirillaceae , light harvesting complex , rhodospirillales , photosynthetic reaction centre , photosynthesis , absorption spectroscopy , photochemistry , extinction (optical mineralogy) , analytical chemistry (journal) , chromatography , electron transfer , biochemistry , photosystem ii , optics , mineralogy , physics , mutant , gene
The molar extinction coefficients of light-harvesting complex 2 (LH2) have been ambiguous in spite of its fame and wide utilization. Herein we determine the molar extinction coefficients of the LH2 proteins derived from the three purple photosynthetic bacteria Rhodoblastus acidophilus, Rhodobacter sphaeroides and Phaeospirillum molischianum at 298 K by direct extraction of bacteriochlorophyll (BChl) a from the lyophilized proteins, followed by estimation of BChl a amounts from their electronic absorption spectra.