Open AccessBODIPY-labeled Fluorescent Aptamer Sensors for Turn-on Sensing of Interferon-gamma and Adenine Compounds on Cells
Author(s) -
Akira Tsuchiya,
Siti Norulhuda Hashim,
Shoko Ise,
Takafumi Furuhata,
Kiyohiko Kawai,
Rie Wakabayashi,
Masahiro Goto,
Noriho Kamiya,
Shinsuke Sando
Publication year - 2016
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.32.543
Subject(s) - aptamer , chemistry , bodipy , fluorescence , molecule , biophysics , combinatorial chemistry , cell , turn (biochemistry) , biochemistry , microbiology and biotechnology , organic chemistry , physics , quantum mechanics , biology
An on-cell aptamer sensor has the potential to reveal cell-cell communications by signalling molecules. We attempted to design new fluorescent aptamer sensors for the sensing of IFN-γ and adenine compounds on cells. BODIPY-labeled external quencher-free aptamer sensors have allowed a turn-on detection of the target molecule with improved off/on efficiency.