Open AccessEvaluation of Enzymatic Activities in Living Systems with Small-molecular Fluorescent Substrate Probes
Author(s) -
Toshimitsu Komatsu,
Yasuteru Urano
Publication year - 2015
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.31.257
Subject(s) - chemistry , substrate (aquarium) , fluorescence , enzyme , fluorescent labelling , substrate specificity , biochemistry , nanotechnology , combinatorial chemistry , biology , ecology , physics , materials science , quantum mechanics
In this review, we aim to present an overview of how small-molecular fluorescent substrate probes for studying enzymatic functions are developed and how they are used in biological applications, under the following four headings: (1) History of Visual Detection of Enzymatic Activities, (2) Strategies to Design Fluorescent Substrate Probes to Measure Enzymatic Activities, (3) Development of Fluorescent Substrate Probes Suitable for Biological Studies, and (4) Biological Applications of Fluorescent Substrate Probes for Studying Enzymes.