Open AccessTube Radial Distribution Chromatography on a Microchip Incorporating Microchannels with a Three-to-One Channel Confluence Point
Author(s) -
Naomichi Suzuki,
Kenichi Yamashita,
Hideaki Maeda,
Masahiko Hashimoto,
Kazuhiko Tsukagoshi
Publication year - 2015
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.31.1267
Subject(s) - chemistry , confluence , chromatography , tube (container) , channel (broadcasting) , distribution (mathematics) , analytical chemistry (journal) , mathematical analysis , mechanical engineering , electrical engineering , mathematics , computer science , engineering , programming language
We developed a capillary chromatography system using a phase-separated solvent mixture as a carrier solution--i.e., a water-hydrophilic/hydrophobic organic solvent mixture--which we call "tube radial distribution chromatography" (TRDC). Here, we attempted to apply the TRDC system to a microchip incorporating microchannels with a double T-junction for injection of analyte solution and a three-to-one, narrow-to-wide channel confluence point for tube radial distribution phenomenon (TRDP) at room temperature. A ternary mixed solvent of water, acetonitrile and ethyl acetate was used as a carrier solution. TRDP in the wide microchannel was examined using various flow rates, temperatures, and component solvent ratios. Successful observation was carried out using a fluorescence microscope-CCD camera. Model analytes perylene (hydrophobic) and Eosin Y (hydrophilic) were separated by flowing through the microchannel, without any treatment such as packed columns or coating, at room temperature (25°C).