Open AccessDetermination of Acid Dissociation Constant of Pravastatin under Degraded Conditions by Capillary Zone Electrophoresis
Author(s) -
Toshio Takayanagi,
Mika Amiya,
Natsumi Shimakami,
Tomoki Yabutani
Publication year - 2015
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.31.1193
Subject(s) - chemistry , capillary electrophoresis , dissociation constant , pravastatin , electrophoresis , chromatography , acid dissociation constant , dissociation (chemistry) , analytical chemistry (journal) , aqueous solution , biochemistry , organic chemistry , receptor , cholesterol
The acid dissociation constant of pravastatin was determined under degraded conditions. Pravastatin was degraded in an acidic solution (pH = 2.0) for 5 h, and the degradation solution was subjected to the measurement of the effective electrophoretic mobility by capillary zone electrophoresis. Although the amount of pravastatin decreased by the acid degradation, its acid dissociation constant was successfully determined with the residual pravastatin through its effective electrophoretic mobility. The determined acid dissociation constant value agreed well with the one obtained with freshly prepared solution and with some reported values.