Open AccessSimple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma
Author(s) -
Hiroya Murakami,
Erina Otani,
Tomoko Iwata,
Yukihiro Esaka,
Takuma Aoyama,
Masanori Kawasaki,
Toshiki Tanaka,
Shinya Minatoguchi,
Bunji Uno
Publication year - 2015
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.31.1189
Subject(s) - chemistry , hydrophilic interaction chromatography , chromatography , ammonium acetate , ultrafiltration (renal) , acetonitrile , adenosine , electrospray ionization , human plasma , high performance liquid chromatography , mass spectrometry , biochemistry
A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.