Open AccessA Fluorescence Probe Based on Biomolecule-stabilized Gold Nanoclusters for the Detection of Pazufloxacin Mesilate
Author(s) -
Xueling Cao,
Lili Lian,
Hongwei Li,
Yuqing Wu,
Dawei Lou
Publication year - 2014
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.30.817
Subject(s) - nanoclusters , chemistry , biomolecule , detection limit , fluorescence , fluorescence spectroscopy , circular dichroism , quenching (fluorescence) , linear range , analytical chemistry (journal) , chromatography , stereochemistry , organic chemistry , optics , biochemistry , physics
In the present study, biomolecule-stabilized Au nanoclusters were demonstrated as a novel fluorescence probe for sensitive and selective detection of pazufloxacin mesilate (PZFX) for the first time. The linear decrease in the fluorescence intensity of Au nanoclusters induced by PZFX allowed for the quantitative detection of PZFX in the range of 0.15 μg/mL to 1 mg/mL, and the detection limit for PZFX was 0.2 μg/mL. Circular dichroism spectroscopy and fluorescence decay studies were then performed to discuss the quenching mechanism. In addition, practical application of the present approach was also demonstrated for real samples, which suggested its great potential for accurate analysis of similar drugs.