Open AccessQuantitative Analysis of D-(+)-Glucose in Fruit Juices Using Diffusion Ordered-1H Nuclear Magnetic Resonance Spectroscopy
Author(s) -
Ruge Cao,
Fusae Komura,
Airi aka,
Takeshi Kato,
Junji Fukumashi,
Toshiro Matsui
Publication year - 2014
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.30.383
Subject(s) - chemistry , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , fructose , spectroscopy , orange juice , quantitative analysis (chemistry) , starch , sucrose , nuclear magnetic resonance , diffusion , deuterium , d glucose , chromatography , stereochemistry , organic chemistry , food science , physics , quantum mechanics , thermodynamics
This study works on D-(+)-glucose quantitative analysis using diffusion ordered-quantitative (1)H nuclear magnetic resonance spectroscopy (DOSY-qNMR), by which an analyte could be distinguished from interferences based upon a characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of D-(+)-glucose in deuterium oxide at 30°C was 5.6 × 10(-10) m(2)/s at a field gradient pulse of between 5.0 × 10(-2) and 3.0 × 10(-1) T/m, distinguished from fructose, sucrose and starch. Good linearity (r(2) = 0.9998) was obtained between D-(+)-glucose (0.5-20.0 g/L) and the ratio of the resonance area of α-C1 proton (5.21 ppm) in D-(+)-glucose to that of the β-C1 proton (5.25 ppm) in D-glucuronic acid (50.0 g/L) as an internal standard. The DOSY-qNMR method was successfully applied to quantify D-(+)-glucose in orange juice (18.3 ± 1.0 g/L), apple juice (26.3 ± 0.4 g/L) and grape juice (45.6 ± 0.6 g/L); the values agreed well with a conventional F-kit glucose method.