A Novel, Universal and Sensitive Lateral-Flow Based Method for the Detection of Multiple Bacterial Contamination in Platelet Concentrations | Zendy

Jidong Wang | Zendy; Xiaohui Wang | Zendy; Yuan Li | Zendy; Shaoduo Yan | Zendy; Qianqian Zhou | Zendy; Bo Gao | Zendy; Jianchun Peng | Zendy; Juan Du | Zendy; Qiuxia Fu | Zendy; Shuaizheng Jia | Zendy; Juankun Zhang | Zendy; Linsheng Zhan | Zendy

Open Access

A Novel, Universal and Sensitive Lateral-Flow Based Method for the Detection of Multiple Bacterial Contamination in Platelet Concentrations

Author(s) -

Jidong Wang,

Xiaohui Wang,

Yuan Li,

Shaoduo Yan,

Qianqian Zhou,

Bo Gao,

Jianchun Peng,

Juan Du,

Qiuxia Fu,

Shuaizheng Jia,

Juankun Zhang,

Linsheng Zhan

Publication year - 2012

Publication title -

analytical sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.392

H-Index - 73

eISSN - 1348-2246

pISSN - 0910-6340

DOI - 10.2116/analsci.28.237

Subject(s) - dipstick , detection limit , chemistry , amplicon , nucleic acid , bacteria , microbiology and biotechnology , polymerase chain reaction , chromatography , fluorescein isothiocyanate , 16s ribosomal rna , genomic dna , contamination , nucleic acid detection , dna , fluorescence , biochemistry , biology , gene , genetics , urine , physics , ecology , quantum mechanics

In the present study, we aimed to develop a nucleic acid lateral-flow method for the rapid and sensitive detection of multiple bacteria that contaminate platelet concentrations (PCs). Polymerase chain reaction (PCR) amplicons were produced by a set of board-range primers that recognize the conserved region of bacteria 16S rDNA, followed by hybridization with both an FITC (fluorescein isothiocyanate)-labelled probe and biotin-labelled probe, and then a nucleic acid lateral-flow dipstick (LFD) assay. The LFD accurately identified 7 species of bacteria, but had no cross-reactivity with human genomic DNA. The limit of detection (LOD) of the LFD assay was as low as 10(1) copies/µL of 16S rDNA for plasmid. In the case of spiked PCs without enrichment, the detection limit of LFD for K. pneumonia was 5 CFU/mL, 6.5 × 10(4) CFU/mL for the S. epidermidis and 35 CFU/mL for P. aeruginosa.

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