Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal | Zendy

Towhid Hossain | Zendy; Takayuki Shibata | Zendy; Tsutomu Kabashima | Zendy; Masaaki Kai | Zendy

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Aptamer-Mediated Chemiluminescence Detection of Prion Protein on a Membrane Using Trimethoxyphenylglyoxal

Author(s) -

Towhid Hossain,

Takayuki Shibata,

Tsutomu Kabashima,

Masaaki Kai

Publication year - 2010

Publication title -

analytical sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.392

H-Index - 73

eISSN - 1348-2246

pISSN - 0910-6340

DOI - 10.2116/analsci.26.645

Subject(s) - chemistry , aptamer , chemiluminescence , detection limit , prion protein , bovine spongiform encephalopathy , transmissible spongiform encephalopathy , protein detection , chromatography , membrane , virology , biochemistry , microbiology and biotechnology , scrapie , nanotechnology , medicine , materials science , disease , pathology , biology

Effective recognition and quantitative analysis of the prion protein are important in drug discovery and diagnosis for prion diseases, such as bovine spongiform encephalopathy and Creutzfeldt-Jakob diseases. We have developed a high-throughput method for a specific and sensitive determination of prion protein on a solid-phase membrane, based on a chemiluminescence reaction of aptamer with 3,4,5-trimethoxyphenylglyoxal. This method using aptamer is facile, inexpensive and convenient for the detection of the prion protein on a membrane, indicating a lower detection limit of ca. 4.2 pmol spot(-1).

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