Isomeric Oligosaccharides Analyses Using Negative-ion Electrospray Ionization Ion Mobility Spectrometry Combined with Collision-induced Dissociation MS/MS | Zendy

Tohru Yamagaki | Zendy; Akihiro Sato | Zendy

Open Access

Isomeric Oligosaccharides Analyses Using Negative-ion Electrospray Ionization Ion Mobility Spectrometry Combined with Collision-induced Dissociation MS/MS

Author(s) -

Tohru Yamagaki,

Akihiro Sato

Publication year - 2009

Publication title -

analytical sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.392

H-Index - 73

eISSN - 1348-2246

pISSN - 0910-6340

DOI - 10.2116/analsci.25.985

Subject(s) - chemistry , collision induced dissociation , ion mobility spectrometry , mass spectrometry , dissociation (chemistry) , electrospray ionization , ion , ionization , tandem mass spectrometry , electrospray , ion mobility spectrometry–mass spectrometry , analytical chemistry (journal) , chemical ionization , chromatography , sample preparation in mass spectrometry , organic chemistry

Ion mobility-mass spectrometry (IM-MS) was applied to analyses of isomeric oligosaccharides linked by alpha1-4, alpha1-6, beta1-3, and beta1-4 glycosyl linkages. Negative-ion electrospray ionization (ESI) quadrupole IM time-of-flight mass spectrometry allowed the combination of IM separation and collision-induced dissociation (CID) MS/MS product ion analysis. Multimer formations of hexa-saccharide linkage isomers differ from each other, and their molecular shapes were analyzed by ion mobility spectrometry (IMS). The product ion spectra of the oligosaccharide isomers were measured by negative-ion CID-MS/MS for each IM separated peak. The spectrum for each isomer was distinct, and their corresponding linkage structures were identified by MS/MS analysis.

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