Open AccessElectrochemical Study of Functionalization on the Surface of a Chitin/Platinum-modified Glassy Carbon Paste Electrode
Author(s) -
Kazuharu Sugawara,
Asako Yugami,
Naohito Terui,
Hideki Kuramitz
Publication year - 2009
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.25.1365
Subject(s) - chemistry , glassy carbon , chitin , electrode , platinum , electrochemistry , surface modification , glucose oxidase , chemical engineering , carbon fibers , inorganic chemistry , polymer chemistry , cyclic voltammetry , organic chemistry , chitosan , composite material , catalysis , composite number , materials science , engineering
To functionalize chitin surfaces using proteins, we developed a glucose oxidase (GOD)-chitin/platinum-modified glassy carbon paste electrode (GCPE) as a model. In a weakly acidic solution, negatively charged GOD were immobilized by the protonated acetylamide groups on chitin. When the electrode was immersed in a solution containing GOD, the enzyme was readily immobilized due to the electrostatic interaction. In addition, measurements were performed using electrodes made with powders of different sizes because sensor performance depends on the particle sizes of glassy carbon powder.