Open AccessDetermination of Lysozyme at the Nanogram Level by a Resonance Light-Scattering Technique with Functionalized CdTe Nanoparticles
Author(s) -
Juan Li,
Xiwen He,
Yunli Wu,
WenYou Li,
Yukui Zhang
Publication year - 2007
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.23.331
Subject(s) - lysozyme , chemistry , cadmium telluride photovoltaics , detection limit , nanoparticle , chromatography , resonance (particle physics) , linear range , analytical chemistry (journal) , light scattering , scattering , nanotechnology , biochemistry , optics , materials science , physics , particle physics
Nanoparticles of cadmium telluride coated with mercaptoacetic acid were prepared in the water phase. Further, an assay of lysozyme with a sensitivity at the nanogram level is proposed. At pH 7.28, lysozyme with positive charges can interact with CdTe nanoparticles. The resonance light-scattering (RLS) signals of functionalized nano-CdTe were greatly enhanced by lysozyme in the region of 300-600 nm, characterized with peaks located at 367, 470 and 533 nm. A linear relationship could be established between the enhanced RLS intensity and the lysozyme concentration in the range of 0.06-4.0 microg mL-1. The limit of detection was 9.5 ng mL-1. The contents of lysozyme were determined with recoveries of 95.6-104.8% and RSD of 1.5-2.3%, respectively. This method is sensitive, rapid, accurate and simple, and provides a new and reliable means for the quantity determination of lysozyme.