Time-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues | Zendy

Xiangqian Li | Zendy; Tieming Huo | Zendy; Xiaogang Chu | Zendy; Chuanlai Xu | Zendy

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Time-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues

Author(s) -

Xiangqian Li,

Tieming Huo,

Xiaogang Chu,

Chuanlai Xu

Publication year - 2007

Publication title -

analytical sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.392

H-Index - 73

eISSN - 1348-2246

pISSN - 0910-6340

DOI - 10.2116/analsci.23.321

Subject(s) - chemistry , aquaculture , microchemistry , chromatography , environmental chemistry , fishery , fish <actinopterygii> , biology

A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of 19-nortestosterone (17beta-NT) residues in aquaculture tissues. The limit of detection (LOD) was determined to be 0.08 ng g-1 and the limit of quantification (LOQ) was less than 0.8 ng g-1. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS). This proposed technique could be applied to routine residue analysis.

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