Open AccessTime-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues
Author(s) -
Xiangqian Li,
Tieming Huo,
Xiaogang Chu,
Chuanlai Xu
Publication year - 2007
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.23.321
Subject(s) - chemistry , aquaculture , microchemistry , chromatography , environmental chemistry , fishery , fish <actinopterygii> , biology
A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of 19-nortestosterone (17beta-NT) residues in aquaculture tissues. The limit of detection (LOD) was determined to be 0.08 ng g-1 and the limit of quantification (LOQ) was less than 0.8 ng g-1. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS). This proposed technique could be applied to routine residue analysis.