Open AccessIndirect Fluorometric Determination of Diclofenac Sodium
Author(s) -
Marcos Castillo,
Liliana Bruzzone
Publication year - 2006
Publication title -
analytical sciences
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.22.431
Subject(s) - chemistry , diclofenac sodium , detection limit , cerium , chromatography , sodium , fluorescence spectrometry , diclofenac , high performance liquid chromatography , fluorescence , inorganic chemistry , organic chemistry , biochemistry , physics , quantum mechanics
A simple and easy method of analysis for diclofenac sodium is reported. A spectrofluorometric method for the microdetermination of diclofenac sodium has been developed through its reaction with cerium(IV) in an acidic solution and measurement of the fluorescence of the Ce(III) ions produced. Under the optimum experimental conditions for the oxidation reaction, 1.0 M H2SO4 with 90 min of heating time (100 degrees C), the range of application is 124.3-600 ng mL(-1) and the limit of detection is 72.7 ng mL(-1). The proposed method was applied to the determination of diclofenac sodium in pharmaceutical tablets. The results of the analysis show a good agreement with those obtained by the official USP 27 HPLC method.