Open AccessFluorescence Lifetime Imaging Microscope Consisting of a Compact Picosecond Dye Laser and a Gated Charge-Coupled Device Camera for Applications to Living Cells
Author(s) -
Tomohiro Uchimura,
Satoshi Kawanabe,
Yuki Maeda,
Totaro Imasaka
Publication year - 2006
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.22.1291
Subject(s) - microscope , fluorescence , picosecond , chemistry , fluorescence microscope , laser , charge coupled device , microscopy , optics , dye laser , resolution (logic) , laser induced fluorescence , optical microscope , optoelectronics , materials science , scanning electron microscope , physics , artificial intelligence , computer science
An inverted microscope was combined with a compact dye laser with a pulse width of <190 ps and an intensified charge-coupled device (ICCD) camera with a minimum gate width of 200 ps. The resulting fluorescence lifetime imaging microscope, which has a temporal resolution of 340 ps, was used to measure the fluorescence lifetime of polymer microspherers. The results indicated a fluorescence lifetime of 0.9 ns. The present analytical instrument was also employed in an evaluation of biological cells after labeling them with SYTO 13, a fluorescent dye.