Open AccessOscillatory Reaction Using Immobilized Catalase
Author(s) -
Satoshi Sasaki,
Yoshikazu Arai
Publication year - 2004
Publication title -
analytical sciences
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.20.895
Subject(s) - chemistry , oscillation (cell signaling) , dialysis , substrate (aquarium) , membrane , catalase , enzyme , immobilized enzyme , briggs–rauscher reaction , chromatography , analytical chemistry (journal) , biochemistry , catalysis , medicine , oceanography , geology
Oscillation is found in many biological systems, and among them the enzymatic oscillatory reaction has been well studied using an enzyme solution. We show in this study for the first time that oscillation occurs when catalase is immobilized to controlled pore glass (CPG). The oscillatory wave mode changes with the distance among the CPG, electrode, or dialysis membrane. The lower substrate concentration results in oscillation with a longer period. This tendency agrees with a previous study using an enzyme solution. Furthermore, we show that the oscillation occurs when no dialysis membrane is used. These results show the wider applicability of the system to analysis or novel device fabrication.