Open AccessSpectrofluorometric Determination of Hydrogen Peroxide Based on Oxidative Catalytic Reactions of p-Hydroxyphenyl Derivatives with Metal Complexes of Thiacalix[4]arenetetrasulfonate on a Modified Anion-Exchanger
Author(s) -
Junichi Odō,
K Matsumoto,
Eri Shinmoto,
Yoko Hatae,
Atsushi Shiozaki
Publication year - 2004
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.20.707
Subject(s) - chemistry , hydrogen peroxide , catalysis , metal , inorganic chemistry , calibration curve , peroxide , metal ions in aqueous solution , nuclear chemistry , detection limit , organic chemistry , chromatography
The peroxidase-like catalytic activity of metal complexes of thiacalix[4]arenetetrasulfonate (TCAS[4]) on a modified anion-exchanger (Me(n+)-TCAS[4]A-500; Me(n+) = H2, Fe3+, Fe2+, Mn3+, Co3+, Co2+, Cu2+, Zn2+, Ni2+) for the oxidation of p-hydroxyphenyl derivatives to produce fluorescent substances in the presence of hydrogen peroxide has been investigated. Among the Me(n+)-TCAS[4]A-500 tested, Fe(3+)-TCAS[4]A-500 exhibited the highest level of catalytic activity for the oxidation of p-acetoamidophenol in a carbonate buffer solution of pH 10. The catalytic activity of Fe(3+)-TCAS[4]A-500 was then used for the spectrofluorometric determination of hydrogen peroxide. The calibration curve for the Fe(3+)-TCAS[4]A-500 method was linear over a range spanning from 0.1 to 5.0 microg of hydrogen peroxide in a 1.0 ml sample solution.