Open AccessDevelopment of the Single-Cell MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) Mass-Spectroscopic Assay
Author(s) -
Masakazu Shimizu,
Noriyuki Ojima,
Hitoshi Ohnishi,
Tomoteru Shingaki,
Yasuyuki Hirakawa,
Tsutomu Masujima
Publication year - 2003
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.2116/analsci.19.49
Subject(s) - chemistry , intracellular , mass spectrometry , pipette , desorption , histamine , matrix assisted laser desorption/ionization , cell , chromatography , mast cell , monolayer , single cell analysis , biophysics , analytical chemistry (journal) , biochemistry , medicine , organic chemistry , adsorption , biology , immunology
MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were estimated.