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Penelitian ini bertujuan mengetahui aktivitas antibakteri ekstrak daun pandan wangi ( Pandanus amaryllifolis roxb ) terhadap Escherichia coli dan Salmonella sp. Esktrak daun pandan dibagi menjadi 3 konsentrasi yang berbeda yaitu 25%, 50%, 75%. Uji aktivitas antibakteri dilakukansesuaidengan metode Kirby-Bauer. Hasil rata-rata zona hambat ekstrak daun pandan wangi terhadap bakteri Escherichia coli pada konsentrasi 25% yaitu 6,6 mm, pada konsentrasi 50% yaitu 6,7 mm, dan pada konsentrasi 75% diperoleh rata-rata 6,9 mm. Zona hambat ekstrak daun pandan wangi terhadap Salmonella sp. pada konsentrasi 25% rata-rata 6,3 mm, pada konsentrasi 50%, 6,5 mm dan pada konsentrasi 75% diperoleh rata-rata 7,3 mm. Kontrol positif antibiotik ampicilin terhadap bakteri Escherichia coli didapatkan rata-rata 6,1 mm, sedangkan pada kontrol positif antibiotik kloramphenikol terhadap bakteri Salmonella sp dengan rata-rata 23,3 mm. Berdasarkan penelitian ini dapat disimpulkan bahwa ekstrak daun pandan wangi mamiliki aktivitas yang lemah dalam menghambat pertumbuhan Escherichia coli dan Salmonella sp. ( The aim s of this research is to know the antibacterial activity of pandanfragnant leaf extract (Pandanusamary llifolis roxb) against Escherichia coli and Salmonella sp. Pandan leaf extract was divided into 3 different concentrations 25%, 50%, 75%. The antibacterial activity test was performed in accordance to K irby- B auer method. The average of inhibition zone of pandanus fragrant leaves extract on Escherichia coli at 25% concentration was 6,6 mm, at concentration of 50% was 6,7 mm, and at concentration 75% obtained average 6,9 mm. The average inhibition zone of pandanus fragrant leaf extract againts Salmonella sp. at 25% concentration was 6.3 mm, at concentrations of 50% was 6.5 mm and at concentration of 75% obtained an average of 7.3 mm. The averaged positive control of ampicillin antibiotics against Escherichia coli was 6.1 mm, the positive control of cloramphenikol antibiotics against Salmonella sp was 23.3 mm. Base on this research it can be concluded that the pandanus fragrant leaf extract had weak activity to inhibit the growth of Escherichia coli and Salmonella sp.)

UJI DAYA HAMBAT EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) TERHADAP BAKTERI Escherichia coli dan Salmonella sp. (Inhibitory Tests of Leaf Extract Pandan Fragrant (Pandanus amaryllifolius Roxb) to Bacteria Escherichia coli and Salmonella sp.)