Application of Micro-kinetic Chromogenic Method in Endotoxin Quantitative Detection of Hydroxypropyl-β-cyclodextrin | Zendy

Lina Yu | Zendy; Yusheng Pei | Zendy

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Application of Micro-kinetic Chromogenic Method in Endotoxin Quantitative Detection of Hydroxypropyl-β-cyclodextrin

Author(s) -

Lina Yu,

Yusheng Pei

Publication year - 2014

Publication title -

journal of applied virology

Language(s) - English

Resource type - Journals

eISSN - 2306-6210

pISSN - 2305-5154

DOI - 10.21092/jav.v3i4.35

Subject(s) - chromogenic , limulus , limulus amebocyte lysate , cyclodextrin , chromatography , lysis , chemistry , biochemistry , biology , immunology , lipopolysaccharide , paleontology

The kinetic chromogenic assay with Limulus amoebocyte lysate to detect the bacterial endotoxin in Hydroxypropyl-β-cyclodextrin for injection is investigated in this paper. The test for interfering factors is performed to study the applicability of the kinetic chromogenic assay for detection of bacterial endotoxin in Hydroxypropyl-β-cyclodextrin for injection. The results suggest that r>0.980 while the range is between 50-0.005EU/ml. Besides, when concentration of test solution is 1mg/ml for Hydroxypropyl-β-cyclodextrin, there is no interference between Limulus amebocyte lysate and bacterial endotoxin. The kinetic chromogenic assay can be used to detect the bacterial endotoxin in Hydroxypropyl- -β-cyclodextrin.

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