Open AccessNon-negligible factors in studying the ApoM-S1P axis using EA.hy926 cells
Author(s) -
Yuanping Shi,
Yun Liang,
Jun Zhang,
Miaomei Yu,
Min Wang,
Lu Zheng,
Dongmei Di,
Xiaoying Zhang,
Guanghua Luo,
Ning Xu
Publication year - 2020
Publication title -
annals of translational medicine
Language(s) - English
Resource type - Journals
eISSN - 2305-5847
pISSN - 2305-5839
DOI - 10.21037/atm.2020.03.74
Subject(s) - cell culture , microbiology and biotechnology , biology , genetics
EA.hy926 cell line is suitable for investigation of the ApoM-S1P axis . However, Since EA.hy926 cells expressed endogenous ApoM, C-myc and His tagged proteins, the exogenous recombinant ApoM should not be labeled with C-myc and His tags for distinguishing from endogenous ApoM. In addition, overexpression of ApoM should be considered to significantly increase the expression of S1PR1 when studying the APOM-S1P axis.
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