Rapid identification of pathogens involved in pediatric osteoarticular infections by multiplex PCR
Author(s) -
Chi Gan,
Jinfeng Hu,
Qing Cao,
Ruike Zhao,
Yuchan Li,
Zhigang Wang,
Yue Tao,
Xi Mo
Publication year - 2020
Publication title -
annals of translational medicine
Language(s) - English
Resource type - Journals
eISSN - 2305-5847
pISSN - 2305-5839
DOI - 10.21037/atm.2020.01.34
Subject(s) - microbiology and biotechnology , multiplex polymerase chain reaction , pseudomonas aeruginosa , staphylococcus aureus , multiplex , primer (cosmetics) , sccmec , biology , streptococcus pyogenes , methicillin resistant staphylococcus aureus , pathogen , polymerase chain reaction , medicine , bacteria , gene , bioinformatics , genetics , chemistry , organic chemistry
This newly developed multiplex PCR assay, without sequencing, enables a rapid and accurate diagnosis of the major bacterial species in children with OAIs and might serve as an additional diagnostic approach for urgent pathogen determination.
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