ISOLATION AND IDENTIFICATION OF TERPENOID COMPOUNDS FROM n-HEXANA EXTRACT OF PERMOT PLANT BRACTS (Passiflora foetida L)
Author(s) -
Maria Dewi Astuti,
Tuti Sriwinarti,
Kamilia Mustikasari
Publication year - 2017
Publication title -
jurnal ilmiah berkala sains dan terapan kimia
Language(s) - English
Resource type - Journals
eISSN - 2549-8215
pISSN - 1411-1616
DOI - 10.20527/jstk.v11i2.4041
Subject(s) - double bond , terpenoid , chemistry , hexane , carbon 13 nmr , stereochemistry , organic chemistry , bract , proton nmr , botany , biology , inflorescence
Isolation and identification of terpenoids compounds from n-hexane extract of permot bracts plants (Passiflora foetida L.) has been done. Crude powder of the bracts permot plants were macerated with n-hexane. Fractionated of n-hexane extract on a vacuum liquid chromatography to afford three fractions (A, B and C). Purification of fraction B with TLC preparative to afford isolate B1 and B2.. A compound of spectra UV-Vis B1 indicating the presence of conjugation of double bond (C=C), furthermore the IR spectra showed vibration for - OH group, C=O of the ester group, C-O group, and methyl group (-CH 3 ) in position gem dimethyl. 1 H-NMR spectra showed the signal from methoxy group (OCH 3 ), olefin protons (-CH=CH-), protons in carbon to the oxygenation (C-OH), two units gem dimethyl, and three methyl group (-CH 3 ). 13 C-NMR spectra showed the signal from carbon aliphatic group, carbon carbonil (C=O) of the ester group, double bond carbon (C=C), and carbon to the oxygenation (C-OCH 3 and C-OH)). Based on the analysis of UV-Vis, IR, 1 H-NMR, and 1 H-NMR spectrocopies data, isolate B1 is suggested from terpenoids type triterpenoids pentacyclic that conjugation of double bond and ester group. Keywords : Terpenoids, triterpenoids pentacyclic, permot (Passiflora foetida L.), and n-hexane extract.
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