HOW DOES QUERCETIN AND TAMOXIFEN AFFECT THE APOPTOSIS OF COLON CANCER CELLS?

KOLON KANSER HUCRELERININ APOPTOZUNU KERSETIN VE TAMOKSIFEN NASIL ETKILER? Ozet:  Diyetle alinan cesitli sebze ve meyvelerde bulunan bilesiklerin kanser tedavisine yardimci olabilecegi bilinmektedir. Polifenolik bir bilesik olan kersetinin de bazi kanserlerde cesitli molekuller uzerinden etkili olabildigi hakkinda mevcut arastirmalar vardir. Ancak etki mekanizmalarinin belirlenmesi icin calismalar devam etmekte olup, tam olarak acikliga kavusmamistir. Tamoksifen ise ostrojen pozitif meme kanserinin tedavisinde kullanilan bir nonsteroid anti-ostrojen ilactir. Zaman zaman meme kanseri disinda diger bazi kanserlerde de kullanildigi hakkinda cesitli bilgilere sahip oldugumuz tamoksifenin, kolon kanserinde de etkili olabilecegini dusunerek arastirmamizda kolon kanser hucrelerinde bu iki bilesenin apoptoz uzerine etkilerini incelemeyi amacladik.  Kersetin ve tamoksifenin 24, 48 ve 72. saatlerde apoptotik indekse olan etkileri TUNEL metodu belirlendi. Kolon kanser hucrelerinde kersetin ve tamoksifenin cesitli konsantrasyonlarda (25, 50 ve 100 µM) apoptotik indekse olan etkileri belirlendi. Bulgular: Apoptotik indeksin kersetin ve tamoksifen uygulanan gruplarda kontrol grubuna gore arttigi bulundu. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. Kolon adeno karsinomada kersetin ve tamoksifenin apoptozu indukleyerek kanser tedavisinde onemli bir rol oynayabilecegini bulunmustur. ANAHTAR KELIMELER: Apoptoz, CaCo-2, Kersetin, Kolon Kanseri, Tamoksifen Abstarcat:  It is a known fact that compounds found in various vegetables and fruits taken by diet may be of help in treating cancer. A polyphenolic compound, quercetin was reported to have a possible impact on some types of cancer through a variety of molecules. However, there are ongoing studies to prove the effect mechanisms and it has yet to be clarified. Tamoxifen is a non-steroidal anti-estrogen drug used in the treatment of estrogen-positive breast cancer. We already have some information regarding occasional use of tamoxifen in treatment of some cancers other than breast cancer, and thus we began investigating the effects of these two components on apoptosis in colon cancer cells in our study considering that it may also be effective in colon cancer. The effect of quercetin and tamoxifen on apoptotic index was evaluated by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method at the 24th, 48th, and 72nd hours. In colon cancer cells, the effects of various concentrations (25, 50, and 100 µM) of quercetin and tamoxifen on apoptotic index in colon cancer cells were established. Apoptotic index increased in groups that were administered quercetin and tamoxifen compared to control groups. The current study shows that quercetin and tamoxifen may play an important role in the treatment of cancer by inducing apoptosis in colon adenocarcinoma. KEYWORD: Apoptosis, CaCo-2, Quercetin, Colon Cancer, Tamoxifen