Open AccessComprehensive Evaluation of the Anti-Helicobacter pylori Activity of Scutellariae Radix
Author(s) -
Ba-Wool Lee,
Il-Ho Park,
Dongsool Yim,
Sung Sook Choi
Publication year - 2017
Publication title -
natural product sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.322
H-Index - 25
eISSN - 2288-9027
pISSN - 1226-3907
DOI - 10.20307/nps.2017.23.1.46
Subject(s) - chrysin , baicalein , wogonin , chemistry , dpph , helicobacter pylori , urease , pharmacology , biochemistry , enzyme , antioxidant , flavonoid , medicine , scutellaria baicalensis , alternative medicine , traditional chinese medicine , pathology
− The aim of this study was to evaluate the anti-Helicobacter pylori activity of fractions and major aglycon compounds (baicalein, chrysin, oroxylin A, wogonin) of Scutellariae Radix. Minimum inhibitory concentration (MIC) measurement, DPPH radical-scavenging assay, DNA protection assay, and urease inhibition analysis were performed. The ethyl acetate (EtOAc) fraction showed the potent anti-Helicobacter activity, and therefore, compounds in the EtOAc fraction were subjected to further assay. The MICs of chrysin, oroxylin A, and wogonin against Helicobacter pylori 26695 were 6.25, 12.5 and 25 μg/mL, respectively. Baicalein exhibited the most effective DPPH radical-scavenging activity. DNA protection using Fenton reaction, chrysin, oroxylin A, and wogonin showed effective DNA protective effect. This result was also confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Regarding Jack bean urease (0.5 mg/mL, 50 unit/mg) inhibition, 20 mM ofbaicalein and chrysin inhibited urease activity by 88.2% and 72.5%, respectively.
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