IN VITRO ANTIBACTERIAL ACTIVITIES OF DIFFERENT EXTRACT OF Moringa oleifera LEAF ON SOME PATHOGENIC BACTERIA | Zendy

K.V. Okolo | Zendy; R.N. Umeji | Zendy; Arthur C. Okafor | Zendy; P.E. Anyiji | Zendy

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IN VITRO ANTIBACTERIAL ACTIVITIES OF DIFFERENT EXTRACT OF Moringa oleifera LEAF ON SOME PATHOGENIC BACTERIA

Author(s) -

K.V. Okolo,

R.N. Umeji,

Arthur C. Okafor,

P.E. Anyiji

Publication year - 2016

Publication title -

american journal of life science researches

Language(s) - English

Resource type - Journals

eISSN - 2375-7485

pISSN - 2332-0206

DOI - 10.20286/ajlsr-040295

Subject(s) - moringa , ethyl acetate , phytochemical , chemistry , antibacterial activity , saponin , salmonella typhi , tannin , traditional medicine , ethanol , agar diffusion test , diethyl ether , enterobacter aerogenes , proteus mirabilis , microbiology and biotechnology , bacteria , food science , biology , escherichia coli , chromatography , biochemistry , medicine , alternative medicine , pathology , genetics , gene

Leaf extracts of Moringa oleifera (aqueous, diethyl ether, ethanol, and ethyl acetate) were tested on Echerichia coli, Pseudomonas aeroginosa, Staphylococcus aureus, Salmonella typhi, Streptococcus pyogenes and Klebsiella aerogenes using agar well diffusion method. Methods: Antibiotics (control) used were tetracycline and ciprofloxacin at 250 mg/mL. Results: Results obtained showed that 200 mg/mL of diethyl ether, ethanol, ethyl acetate and 100% aqueous extracts had more effect than 160 mg/mL of diethyl ether, ethanol, ethyl acetate and 80% aqueous respectively. The results showed that Salmonella typhi was more susceptible than other organisms and ethyl acetate extract was more effective than other extracts. The phytochemical screening indicated the presence of flavonoid, alkaloids, tannin, glycoside, saponin, and phenol. Conclusions: The study showed that Moringa oleifera can be used as potential herbs for treatment of bacteria pathogens tested in this study and these activities could be attributed to the presence of these secondary metabolites. © 2016. American Journal of Life Science Researches. Submited: 14.12.2015 Accepted: 23.03.2016

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